Esbona K, Yi Y, Saha S, Yu M, Van Doorn RR, Conklin MW, Graham DS, Wisinski KB, Ponik SM, Eliceiri KW, Wilke LG, Keely PJ. The presence of COX-2, tumor-associated macrophages, and collagen alignment as prognostic markers for invasive breast carcinoma patients. Am J Pathol 2018, 188: 559-573

Ishihara S, Inman DR, Li W, Ponik SM, Keely PJ. Mechano-signal transducation of mesenchymal stem cells induce mammary cancer growth via prosaposin secretion.  Cancer Res. 2017 Sep 28 PMID:28972074

Morris BA, Burkel B, Ponik SM, Fan J, Condeelis JS, Aguire-Ghiso JA, Castracane J, Denu JM, Keely PJ. Collagen Matrix Density Drives the Metabolic Shift in Breast Cancer Cells. EBioMedicine. 2016 Nov;13:146-156. doi: 10.1016/j.ebiom.2016.10.012.

Szulczewski JM, Inman DR, Entenberg D, Ponik SM, Aguirre-Ghiso J, Castracane J, Condeelis J, Eliceiri KW, Keely PJ. In Vivo Visualization of Stromal Macrophages via label-free FLIM-based metabolite imaging. Sci Rep. 2016 May 25;6:25086. doi: 10.1038/srep25086.

Burkel B, Morris BA, Ponik SM, Riching KM, Eliceiri KW, Keely PJ. Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo. J Vis Exp. 2016 May 9;(111). doi: 10.3791/53989. PubMed PMID: 27213771
García-Mendoza MG, Inman DR, Ponik SM, Jeffery JJ, Sheerar DS, Van Doorn RR, Keely PJ. Neutrophils drive accelerated tumor progression in the collagen-dense mammary tumor microenvironment. Breast Cancer Res. 2016 May 11;18(1):49. doi:
10.1186/s13058-016-0703-7. PubMed PMID: 27169366

Esbona K, Inman D, Saha S, Jeffery J, Schedin P, Wilke L, Keely P. COX-2 modulates mammary tumor progression in response to collagen density. Breast Cancer Res. 2016 Mar 22;18(1):35. doi: 10.1186/s13058-016-0695-3. PMID: 27000374

Curran CS, Carrillo ER, Ponik SM, Keely PJ. Collagen density regulates xenobiotic and hypoxic response of mammary epithelial cells. Environ Toxicol Pharmacol. 2015 Jan;39(1):114-24. doi: 10.1016/j.etap.2014.10.017. Epub 2014 Nov 1. PubMed PMID: 25481308.
Riching KM, Keely PJ. Rho family GTPases: making it to the third dimension. Int J Biochem Cell Biol. 2015 Feb;59:111-5. doi: 10.1016/j.biocel.2014.11.007. Epub 2014 Dec 3. Review. PubMed PMID: 25478651.
Riching KM, Cox BL, Salick MR, Pehlke C, Riching AS, Ponik SM, Bass BR, Crone WC, Jiang Y, Weaver AM, Eliceiri KW, Keely PJ. 3D collagen alignment limits protrusions to enhance breast cancer cell persistence. Biophys J. 2014 Dec 2;107(11):2546-58. doi: 10.1016/j.bpj.2014.10.035. Epub 2014 Dec 2. PubMed PMID:
Lee B, Zhou X, Riching K, Eliceiri KW, Keely PJ, Guelcher SA, Weaver AM, Jiang Y. A three-dimensional computational model of collagen network mechanics. PLoS One. 2014 Nov 11; 9(11):e111896. doi: 10.1371/journal.pone.0111896. eCollection
2014. PubMed PMID: 25386649.
Bredfeldt JS, Liu Y, Conklin MW, Keely PJ, Mackie TR, Eliceiri KW. Automated quantification of aligned collagen for human breast carcinoma prognosis. J Pathol Inform. 2014 Aug 28;5:28. doi: 10.4103/2153-3539.139707. eCollection 2014. PubMed
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Bredfeldt JS, Liu Y, Pehlke CA, Conklin MW, Szulczewski JM, Inman DR, Keely PJ, Nowak RD, Mackie TR, Eliceiri KW. Computational segmentation of collagen fibers from second-harmonic generation images of breast cancer. J Biomed Opt. 2014 Jan;19(1):16007. doi: 10.1117/1.JBO.19.1.016007. PubMed PMID: 24407500.
Keely PJ. Proteolytic remodeling of the ECM and the geometric control of stem cell fate. Dev Cell. 2013 May 28;25(4):325-6. doi: 10.1016/j.devcel.2013.05.012. PubMed PMID: 23725758.
Hollenbeck S, Keely PJ, Seewaldt V. Mammographic density: intersection of science, the law, and clinical practice. Am Soc Clin Oncol Educ Book. 2013. doi: 10.1200/EdBook_AM.2013.33.e63. PubMed PMID: 23714458.
Zhang K, Corsa CA, Ponik SM, Prior JL, Piwnica-Worms D, Eliceiri KW, Keely PJ, Longmore GD. The collagen receptor discoidin domain receptor 2 stabilizes SNAIL1 to facilitate breast cancer metastasis. Nat Cell Biol. 2013 Jun;15(6):677-87. doi: 10.1038/ncb2743. Epub 2013 May 5. PubMed PMID: 23644467.
Pugh TD, Conklin MW, Evans TD, Polewski MA, Barbian HJ, Pass R, Anderson BD, Colman RJ, Eliceiri KW, Keely PJ, Weindruch R, Beasley TM, Anderson RM. A shift in energy metabolism anticipates the onset of sarcopenia in rhesus monkeys. Aging Cell. 2013 Aug;12(4):672-81. doi: 10.1111/acel.12091. Epub 2013 May 27. PubMed PMID: 23607901.
Gehler S, Ponik SM, Riching KM, Keely PJ. Bi-directional signaling: extracellular matrix and integrin regulation of breast tumor progression. Crit Rev Eukaryot Gene Expr. 2013;23(2):139-57. Review. PubMed PMID: 23582036.
Ponik SM, Trier SM, Wozniak MA, Eliceiri KW, Keely PJ. RhoA is down-regulated at cell-cell contacts via p190RhoGAP-B in response to tensional homeostasis. Mol Biol Cell. 2013 Jun;24(11):1688-99, S1-3. doi: 10.1091/mbc.E12-05-0386. Epub 2013 Apr 3. PubMed PMID: 23552690.
Curran CS, Keely PJ. Breast tumor and stromal cell responses to TGF-β and hypoxia in matrix deposition. Matrix Biol. 2013 Mar 11;32(2):95-105. doi: 10.1016/j.matbio.2012.11.016. Epub 2012 Dec 20. Review. PubMed PMID: 23262216.
Heck JN, Ponik SM, Garcia-Mendoza MG, Pehlke CA, Inman DR, Eliceiri KW, Keely PJ. Microtubules regulate GEF-H1 in response to extracellular matrix stiffness. Mol Biol Cell. 2012 Jul;23(13):2583-92. doi: 10.1091/mbc.E11-10-0876. Epub 2012 May 16. PubMed PMID: 22593214.
Ajeti V, Nadiarnykh O, Ponik SM, Keely PJ, Eliceiri KW, Campagnola PJ. Structural changes in mixed Col I/Col V collagen gels probed by SHG microscopy: implications for probing stromal alterations in human breast cancer. Biomed Opt Express. 2011 Aug 1;2(8):2307-16. doi: 10.1364/BOE.2.002307. Epub 2011 Jul 20. PubMed PMID: 21833367.
Keely PJ. Mechanisms by which the extracellular matrix and integrin signaling act to regulate the switch between tumor suppression and tumor promotion. J Mammary Gland Biol Neoplasia. 2011 Sep;16(3):205-19. doi: 10.1007/s10911-011-9226-0. Epub 2011 Aug 7. Review. PubMed PMID: 21822945.
Lyons TR, O'Brien J, Borges VF, Conklin MW, Keely PJ, Eliceiri KW, Marusyk A,Tan AC, Schedin P. Postpartum mammary gland involution drives progression of ductal carcinoma in situ through collagen and COX-2. Nat Med. 2011 Aug 7;17(9):1109-15. doi: 10.1038/nm.2416. PubMed PMID: 21822285.
Conklin MW, Eickhoff JC, Riching KM, Pehlke CA, Eliceiri KW, Provenzano PP, Friedl A, Keely PJ. Aligned collagen is a prognostic signature for survival in human breast carcinoma. Am J Pathol. 2011 Mar;178(3):1221-32. doi: 10.1016/j.ajpath.2010.11.076. PubMed PMID: 21356373.
Sung KE, Yang N, Pehlke C, Keely PJ, Eliceiri KW, Friedl A, Beebe DJ. Transition to invasion in breast cancer: a microfluidic in vitro model enables examination of spatial and temporal effects. Integr Biol (Camb). 2011 Apr;3(4):439-50. doi: 10.1039/c0ib00063a. Epub 2010 Dec 7. PubMed PMID: 21135965.
Schedin P, Keely PJ. Mammary gland ECM remodeling, stiffness, and mechanosignaling in normal development and tumor progression. Cold Spring Harb Perspect Biol. 2011 Jan 1;3(1):a003228. doi: 10.1101/cshperspect.a003228. Review. PubMed PMID: 20980442.
Provenzano PP, Eliceiri KW, Inman DR, Keely PJ. Engineering three-dimensional collagen matrices to provide contact guidance during 3D cell migration. Curr Protoc Cell Biol. 2010 Jun;Chapter 10:Unit 10.17. doi:10.1002/0471143030.cb1017s47. PubMed PMID: 20521230.
Rueden CT, Conklin MW, Provenzano PP, Keely PJ, Eliceiri KW. Nonlinear optical microscopy and computational analysis of intrinsic signatures in breast cancer. Conf Proc IEEE Eng Med Biol Soc. 2009;2009:4077-80. doi: 10.1109/IEMBS.2009.5334523. PubMed PMID: 19964821.
Provenzano PP, Inman DR, Eliceiri KW, Keely PJ. Matrix density-induced mechanoregulation of breast cell phenotype, signaling and gene expression through a FAK-ERK linkage. Oncogene. 2009 Dec 10;28(49):4326-43. doi: 10.1038/onc.2009.299. Epub . PubMed PMID: 19826415.
Sung KE, Su G, Pehlke C, Trier SM, Eliceiri KW, Keely PJ, Friedl A, Beebe DJ. Control of 3-dimensional collagen matrix polymerization for reproducible human mammary fibroblast cell culture in microfluidic devices. Biomaterials. 2009 Sep;30(27):4833 41. doi: 10.1016/j.biomaterials.2009.05.043. Epub 2009 Jun 21. PubMed PMID: 19540580.
Gehler S, Baldassarre M, Lad Y, Leight JL, Wozniak MA, Riching KM, Eliceiri KW, Weaver VM, Calderwood DA, Keely PJ. Filamin A-beta1 integrin complex tunes epithelial cell response to matrix tension. Mol Biol Cell. 2009 Jul;20(14):3224-38. doi: 10.1091/mbc.E08-12-1186. Epub 2009 May 20. PubMed PMID: 19458194.
Provenzano PP, Eliceiri KW, Yan L, Ada-Nguema A, Conklin MW, Inman DR, Keely PJ. Nonlinear optical imaging of cellular processes in breast cancer. Microsc Microanal. 2008 Dec;14(6):532-48. doi: 10.1017/S1431927608080884. PubMed PMID:18986607.
Provenzano PP, Inman DR, Eliceiri KW, Trier SM, Keely PJ. Contact guidance mediated three-dimensional cell migration is regulated by Rho/ROCK-dependent matrix reorganization. Biophys J. 2008 Dec;95(11):5374-84. doi: 10.1529/biophysj.108.133116. Epub 2008 Sep 5. PubMed PMID: 18775961.
Provenzano PP, Eliceiri KW, Keely PJ. Multiphoton microscopy and fluorescence lifetime imaging microscopy (FLIM) to monitor metastasis and the tumor microenvironment. Clin Exp Metastasis. 2009;26(4):357-70. doi: 10.1007/s10585-008-9204-0. Epub 2008 Sep 3. Review. PubMed PMID: 18766302.
Provenzano PP, Rueden CT, Trier SM, Yan L, Ponik SM, Inman DR, Keely PJ, Eliceiri KW. Nonlinear optical imaging and spectral-lifetime computational analysis of endogenous and exogenous fluorophores in breast cancer. J Biomed Opt. 2008 May-Jun;13(3):031220. doi: 10.1117/1.2940365. PubMed PMID: 18601544.
Provenzano PP, Inman DR, Eliceiri KW, Knittel JG, Yan L, Rueden CT, White JG, Keely PJ. Collagen density promotes mammary tumor initiation and progression. BMC Med. 2008 Apr 28;6:11. doi: 10.1186/1741-7015-6-11. PubMed PMID: 18442412.
Provenzano PP, Eliceiri KW, Campbell JM, Inman DR, White JG, Keely PJ. Collagen reorganization at the tumor-stromal interface facilitates local invasion. BMC Med. 2006 Dec 26;4(1):38. PubMed PMID: 17190588.
Modzelewska K, Newman LP, Desai R, Keely PJ. Ack1 mediates Cdc42-dependentcell migration and signaling to p130Cas. J Biol Chem. 2006 Dec 8;281(49):37527-35. Epub 2006 Oct 12. PubMed PMID: 17038317.
Wozniak MA, Keely PJ. Use of three-dimensional collagen gels to study mechanotransduction in T47D breast epithelial cells. Biol Proced Online. 2005;7:144-61. Epub 2005 Oct 17. PubMed PMID: 16299584.
Wozniak MA, Kwong L, Chodniewicz D, Klemke RL, Keely PJ. R-Ras controls membrane protrusion and cell migration through the spatial regulation of Rac and Rho. Mol Biol Cell. 2005 Jan;16(1):84-96. Epub 2004 Nov 3. PubMed PMID: 15525681.
Wozniak MA, Desai R, Solski PA, Der CJ, Keely PJ. ROCK-generated contractility regulates breast epithelial cell differentiation in response to the physical properties of a three-dimensional collagen matrix. J Cell Biol. 2003 Nov 10;163(3):583-95. PubMed PMID: 14610060.
Kwong L, Wozniak MA, Collins AS, Wilson SD, Keely PJ. R-Ras promotes focal adhesion formation through focal adhesion kinase and p130(Cas) by a novel mechanism that differs from integrins. Mol Cell Biol. 2003 Feb;23(3):933-49. PubMed PMID: 12529399.
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Saelman EU, Keely PJ, Santoro SA. Loss of MDCK cell alpha 2 beta 1 integrin expression results in reduced cyst formation, failure of hepatocyte growth factor/scatter factor-induced branching morphogenesis, and increased apoptosis. J Cell Sci. 1995 Nov;108 ( Pt 11):3531-40. PubMed PMID: 8586664.
Keely PJ, Wu JE, Santoro SA. The spatial and temporal expression of the alpha2 beta 1 integrin and its ligands, collagen I, collagen IV, and laminin, suggest important roles in mouse mammary morphogenesis. Differentiation. 1995 Jul;59(1):1-13. PubMed PMID: 7589890.
Keely PJ, Fong AM, Zutter MM, Santoro SA. Alteration of collagen-dependent adhesion, motility, and morphogenesis by the expression of antisense alpha 2 integrin mRNA in mammary cells. J Cell Sci. 1995 Feb;108 ( Pt 2):595-607. PubMed PMID: 7769004.
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